Neurons reach out to touch a neighbour

These mouse neurons developed in vitro from embryonic stem cells. This image is actually stitched together from 16 separate images taken over 10 days as the formation of a neural network between the cells was tracked. A semi-automated method allows the same area to be followed as cells move or remodel over time, and also for image slices to be taken at different focal depths.
Left: a 1.2 mm x 1.1 mm brightfield image reconstructed from 16 single 3D-stacks (z-projection of 4x4x18 images, 20x magnification). Scale bar length is 250 µm (left) or 50 µm (right). Image credit: Johannes Jung/Angela Garding, Tiwari group at IMB.